Facsaria 2 manual

 · The BD FACSAria cell sorter sets a new standard for high-performance flow cytometry. Based on an entirely new design in instrumentation, the BD FACSAria instrument is the first benchtop sorter that incorporates a fixed-alignment cuvette flow cell. The numerous technological advances embodied in the BD FACSAria cell sorter reduce the cost of. The BD FACSAria II system was set up for a sort using either a μm or μm nozzle (70 psi or 35 psi with a frequency of 87 or 60 kHz respectively). CD45RA+ Tregs and CD45RA-Tregs were sorted in purity mode at a rate of 10, to 11, events per second. Fluidics cart All Events Lymphocyte Gate FSC-A. FACSAria II Sample Preparation Acceptable sample tubes are polypropylene 12 × 75 mm, mL tubes, bullet tubes, or mL microcentrifuge tubes. Concentration should be 10–20 × 10 6 cells per mL (allows for ideal pressure and best use of time).

FACSAria II Sample Preparation Acceptable sample tubes are polypropylene 12 × 75 mm, mL tubes, bullet tubes, or mL microcentrifuge tubes. Concentration should be 10–20 × 10 6 cells per mL (allows for ideal pressure and best use of time). BD FACSAria I Operation Manual 2 Stream Break-Off www.doorway.ru following steps are prepared for droplet formation using µm nozzle. www.doorway.ru ON the Stream (if not yet done), and allow time for the stream to pressurize and settle. www.doorway.ru the Frequency (Freq) between 29 and 32 to identify the HIGHEST break-off it can generate. 1) A 2 hour introduction to the FACSAria II with a practice bead sort. This will include stopping the stream, removing the nozzle, sonicating and reinserting it, restarting the stream and returning to the original drop position. This is an “Introduction to fixing a clog”. Please review the training manual prior to this session.

The BD FACSCanto II Instructions for Use assumes you have a working knowledge of basic Microsoft® Windows® operation. suspension for flow cytometry. This guide contains instructions for using BD FACSDiva™ software version with Verify that the FSC, SSC, and threshold settings are appropriate. 1. 2. The cuvette flow cell is gel-coupled to the fluorescent objective lens to transmit the greatest amount of light to the collection optics. (see Figure 2). The.